Chlorogenic acid in preventing and curing ultraviolet-induced damage in human skin fibroblast as an antiaging cell model

Continuous ultraviolet (UV) irradiation stimulates the over-production of reactive oxygen species (ROS) to cause degenerative diseases. Chlorogenic acid (CA) is found as plants antioxidant that promises medicinal effects. This study examined CA protection against UV-damage in human skin fibroblast (BJ) cells both for curative and preventive therapy. BJ were exposed UV radiation addition (6.26-100 mikro g/mL) by methods. The viability analysis was conducted employing MTS (3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxy-phenyl)-2-(4-sulfophenyl)-2H-tetrazolium) assay. treatment before exposure exhibited an increased percentage than positive control. In detail, series concentration (6.25, 12.5, 25 significantly enhanced viable cells. after denoted same results. Furthermore, lower concentrations used, higher cell resulted. at dose 6.25 g/mL showed highest cells, while 100 resulted lowest viability. short, can preserve treat from exposure. outcome suggested prevention on UV-induced tested applicable further experiments.